Galina L, Pijoan C, Sitjar M, Christianson W T, Rossow K, Collins J E
Department of Clinical and Population Sciences, University of Minnesota, St Paul 55108.
Vet Rec. 1994 Jan 15;134(3):60-4. doi: 10.1136/vr.134.3.60.
Secondary specific pathogen-free (sSPF) piglets were inoculated intranasally with Streptococcus suis serotype 2 alone, porcine reproductive and respiratory syndrome virus (PRRSV) alone, or with PRRSV followed by S suis. Uninfected piglets were used as controls. Pigs inoculated with PRRSV (ATCC VR-2332) followed by challenge with a virulent strain (87555) of S suis serotype 2 developed clinical signs, suppurative meningitis and large numbers of S suis in their tissues, including the brain and meninges. Pigs inoculated with PRRSV alone, S suis (87555) alone, or with PRRSV and the DH5 strain of S suis serotype 2 (lacking a protein associated with virulence) and the uninfected piglets did not develop clinical signs or lesions or have large numbers of bacteria in their tissues. The results suggest that PRRSV predisposes sSPF pigs to infection and disease caused by virulent S suis serotype 2. Co-infection of piglets with PRRSV and a virulent strain of S suis may provide a useful model for the study of S suis septicaemia and meningitis.
将二级无特定病原体（sSPF）仔猪分别经鼻内接种猪链球菌2型、单独接种猪繁殖与呼吸综合征病毒（PRRSV），或先接种PRRSV再接种猪链球菌。未感染的仔猪用作对照。先接种PRRSV（ATCC VR - 2332），再用猪链球菌2型强毒株（87555）攻毒的仔猪出现临床症状、化脓性脑膜炎，其组织（包括脑和脑膜）中有大量猪链球菌。单独接种PRRSV、单独接种猪链球菌（87555）、接种PRRSV和猪链球菌2型DH5菌株（缺乏与毒力相关的蛋白质）以及未感染的仔猪均未出现临床症状或病变，其组织中也没有大量细菌。结果表明，PRRSV使sSPF猪易感染由猪链球菌2型强毒株引起的感染和疾病。仔猪PRRSV与猪链球菌强毒株的共同感染可能为研究猪链球菌败血症和脑膜炎提供有用的模型。
