Mazzei M, Balbi A, Grandi T, Sottofattori E, Garzoglio R, Abramova T, Ivanova E
Istituto di Scienze Farmaceutiche, Genova, Italy.
Farmaco. 1993 Dec;48(12):1649-61.
Antisense oligodeoxynucleotides (ODNs) and their derivatives are highly interesting tools to regulate gene expression and promising drugs for antiviral and anticancer therapy. In view of performing more extensive pharmacological trials requiring relatively great amounts of ODNs, we used a method of ODN synthesis derived from the phosphotriester approach which allow to prepare ODNs covalently linking cholesteryl residue (in 5' or 3') and phenazinium group (in 5'). HPLC purifications are discussed.
反义寡脱氧核苷酸（ODNs）及其衍生物是调节基因表达的极具吸引力的工具，也是抗病毒和抗癌治疗中很有前景的药物。鉴于要进行需要相对大量ODNs的更广泛的药理试验，我们采用了一种源自磷酸三酯法的ODN合成方法，该方法能够制备在5'或3'位共价连接胆固醇残基以及在5'位连接吩嗪鎓基团的ODNs。文中还讨论了高效液相色谱纯化方法。
