视黄酸受体在大鼠、小鼠和人类卵巢及子宫中的免疫定位。

Immunolocalization of retinoic acid receptors in rat, mouse and human ovary and uterus.

作者信息

Zhuang Y H, Ylikomi T, Lindfors M, Piippo S, Tuohimaa P

机构信息

Department of Biomedical Sciences, University of Tampere, Finland.

出版信息

J Steroid Biochem Mol Biol. 1994 Jan;48(1):61-8. doi: 10.1016/0960-0760(94)90251-8.

DOI:10.1016/0960-0760(94)90251-8

PMID:8136307

Abstract

We raised an antibody against a synthetic peptide corresponding to amino acids 155-174 of human retinoic acid receptor alpha (RAR-alpha). The sequence is highly homologous in all RARs and their isoforms. When mouse and human RARs (alpha, beta and gamma) expressed in Cos cell were analysed with immunoblot, all receptors gave a specific 51 K signal. Mouse RAR-gamma gave an additional signal corresponding to 58 K. In human teratocarcinoma cells (F9) both 51 and 58K molecule sizes were detected. The RAR expression in F9 cells was slightly down-regulated in charcoal-stripped culture medium and returned to normal level after retinoic acid treatment. The 51 K protein was found in all ovarian and uterine samples, but the quantity of the 58 K protein varied in different species and organs, being highest in the mouse uterus and the rat and human ovary. Using immunohistochemistry the RARs were found in the nuclear compartment. In the rat uterus, positive immunoreaction was found mainly in the nuclei of epithelial, uterine glandular and stromal cells. In the rat ovary, positive reaction was found in the nuclei of germinal epithelial, follicular and stromal cells.

摘要

我们制备了一种针对与人类视黄酸受体α（RAR-α）第155 - 174位氨基酸对应的合成肽的抗体。该序列在所有RAR及其异构体中高度同源。当用免疫印迹法分析在Cos细胞中表达的小鼠和人类RAR（α、β和γ）时，所有受体都产生了一个特定的51K信号。小鼠RAR-γ还产生了一个对应于58K的额外信号。在人类畸胎瘤细胞（F9）中检测到了51K和58K两种分子大小的信号。F9细胞中的RAR表达在经活性炭处理的培养基中略有下调，在视黄酸处理后恢复到正常水平。在所有卵巢和子宫样本中都发现了51K蛋白，但58K蛋白的量在不同物种和器官中有所不同，在小鼠子宫以及大鼠和人类卵巢中含量最高。通过免疫组织化学方法发现RAR存在于细胞核中。在大鼠子宫中，阳性免疫反应主要出现在上皮细胞、子宫腺细胞和基质细胞的细胞核中。在大鼠卵巢中，在生发上皮细胞、卵泡细胞和基质细胞的细胞核中发现了阳性反应。