Brigotti M, Lorenzetti R, Denaro M, Carnicelli D, Montanaro L, Sperti S
Department of Experimental Pathology, University of Bologna, Italy.
Biochem Mol Biol Int. 1993 Dec;31(5):897-903.
Oligodeoxynucleotides complementary to the alpha-sarcin domain of rat 28S rRNA inhibit cell-free protein synthesis. The poly(U) translation system containing Artemia salina ribosomes was more sensitive to inhibition than the system containing rat liver ribosomes. The 21-mer, which was the most effective of the 7 oligonucleotides tested, hybridized with naked 28S rRNA. Hybridization with whole ribosomes, assayed by S1 nuclease protection, occurred only at high ionic strength or with ribosomes actively engaged in protein synthesis.
与大鼠28S rRNA的α-肌动蛋白结构域互补的寡脱氧核苷酸可抑制无细胞蛋白质合成。含有卤虫核糖体的聚（U）翻译系统比含有大鼠肝脏核糖体的系统对抑制更敏感。在测试的7种寡核苷酸中最有效的21聚体与裸露的28S rRNA杂交。通过S1核酸酶保护测定，与完整核糖体的杂交仅在高离子强度下或与积极参与蛋白质合成的核糖体发生。
