Lu B, Li Q, Liu W Y
Shanghai Institute of Biochemistry, Academia Sinica, China.
Biol Chem. 1997 Jul;378(7):697-9. doi: 10.1515/bchm.1997.378.7.697.
In the static state of the ribosome, its S/R domain is not accessible to a probe of 1 4-mer oligodeoxyribonucleotide complementary to the region. However, during the in vitro translation process, the probe can specifically hybridize with the region in ribosome and inhibit translation. It appears that the S/R domain is well-protected when the ribosome is in the static state. This site is partially exposed at some stage(s) of translation. These results indicate that the S/R domain is not a simple single-strand, but a reversibly dynamic structure.
在核糖体的静态状态下，与其该区域互补的14聚体寡脱氧核糖核苷酸探针无法接近其S/R结构域。然而，在体外翻译过程中，该探针可与核糖体中的该区域特异性杂交并抑制翻译。核糖体处于静态时，S/R结构域似乎受到良好保护。在翻译的某些阶段，该位点会部分暴露。这些结果表明，S/R结构域不是简单的单链，而是一种可逆的动态结构。
