Renard J, Vidal-Madjar C
Laboratoire de Physico-Chimie des Biopolymères, CNRS, Université Paris Val de Marne, Thiais, France.
J Chromatogr A. 1994 Feb 11;661(1-2):35-42. doi: 10.1016/0021-9673(94)85174-3.
The split-peak effect was used to determine the association rate constant of the antigen-immobilized antibody reaction. The amount of immobilized human serum albumin antibody on the chromatographic support was varied in order to find the optimal conditions to reduce the mass transfer contribution in the stagnant mobile phase fluid and measure the effective association rate constant of human serum albumin with the immobilized antibody. Kinetic studies as a function of flow-rate demonstrate the validity of the method consisting in determining the association rate constant from measurements performed on columns of various capacities. These experiments show that limitations due to mass transfer to the surface of the adsorbent are minimized at high flow-rates and for a low density of immobilized ligand.
采用裂峰效应来确定固定化抗原-抗体反应的缔合速率常数。改变色谱载体上固定化人血清白蛋白抗体的量，以找到最佳条件，减少停滞流动相流体中的传质贡献，并测量人血清白蛋白与固定化抗体的有效缔合速率常数。作为流速函数的动力学研究证明了该方法的有效性，即通过在不同容量的柱上进行测量来确定缔合速率常数。这些实验表明，在高流速和低固定化配体密度下，传质到吸附剂表面所造成的限制可降至最低。
