Lawrence J F, Cleroux C, Truelove J F
Bureau of Chemical Safety, Food Directorate, Banting Research Centre, Ottawa, Ontario, Canada.
J Chromatogr A. 1994 Feb 18;662(1):173-7. doi: 10.1016/0021-9673(94)85307-X.
A reversed-phase liquid chromatographic method employing UV absorption detection at 242 nm was compared to a radioimmunoassay technique for the determination of the marine toxin, domoic acid, in several types of seafood and biological samples. Agreement between the two methods for spiked samples of mussels and rat serum was very good over a range of concentrations of 0.15-7.3 micrograms/g domoic acid. Also, a very good correlation was observed between the two methods for naturally incurred residues of domoic acid in razor clams, anchovies and crab meat over a concentration range of 0.6-43 micrograms/g domoic acid.
将一种采用242 nm紫外吸收检测的反相液相色谱法与一种放射免疫分析技术进行了比较，以测定几种类型的海鲜和生物样品中的海洋毒素——软骨藻酸。在软骨藻酸浓度为0.15 - 7.3微克/克的范围内，两种方法对添加了软骨藻酸的贻贝和大鼠血清样品的测定结果非常吻合。此外，在软骨藻酸浓度为0.6 - 43微克/克的范围内，两种方法对自然存在于蛏子、凤尾鱼和蟹肉中的软骨藻酸残留量的测定结果也具有很好的相关性。
