Slayter M V, Anzano M A, Kadomatsu K, Smith J M, Sporn M B
Department of Veterinary Pathology, Armed Forces Institute of Pathology, Washington, DC 20306.
Cancer Res. 1994 Mar 15;54(6):1440-5.
We have developed a grading system for the evaluation of the histogenesis of neoplastic lesions of the prostate and seminal vesicle of the laboratory rat. Prostatic and seminal vesicle carcinomas were induced in Lobund-Wistar rats by initiation with 30 mg/kg N-nitroso-N-methylurea i.v., followed by promotion with 40 mg testosterone propionate implants 1 week later and at 3-month intervals thereafter. Experimental and control groups were sacrificed at various time points between 5 and 11 months after dosing with N-nitroso-N-methylurea in order to visualize progressive stages of carcinogenesis of the dorsolateral prostate, the anterior prostate, and the seminal vesicle. A system of staging was created which allows three different categories (in situ change, invasion, desmoplasia) of tumor development to be ranked progressively in a manner conducive to nonparametric analysis. Each category was then further subdivided to create a total of six stages. This system can be used to evaluate agents which modify tumor induction or suppression. The application of this staging system to the measurement of the effects of the synthetic retinoid, 4-hydroxyphenyl retinamide, on prostatic carcinogenesis in the Lobund-Wistar rat is described.
我们已经开发出一种分级系统，用于评估实验大鼠前列腺和精囊肿瘤性病变的组织发生情况。通过静脉注射30mg/kg N-亚硝基-N-甲基脲对Lobund-Wistar大鼠进行启动，1周后植入40mg丙酸睾酮，此后每隔3个月植入一次，从而诱发前列腺癌和精囊癌。在静脉注射N-亚硝基-N-甲基脲后的5至11个月内的不同时间点处死实验组和对照组，以便观察背外侧前列腺、前列腺前部和精囊致癌作用的进展阶段。创建了一个分期系统，该系统允许将肿瘤发展的三种不同类别（原位改变、浸润、促结缔组织增生）以有利于非参数分析的方式逐步排序。然后将每个类别进一步细分，总共创建六个阶段。该系统可用于评估改变肿瘤诱导或抑制的药物。本文描述了该分期系统在测量合成类视黄醇4-羟基苯基视黄酰胺对Lobund-Wistar大鼠前列腺癌发生影响方面的应用。
