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布鲁氏菌抗体与小肠结肠炎耶尔森菌O:9型抗体血清学鉴别初步研究

[A preliminary study on serological differentiation between antibody against Brucella and that against Yersinia enterocolitica serotype O: 9].

作者信息

Lu Q

机构信息

Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Beijing.

出版信息

Zhonghua Liu Xing Bing Xue Za Zhi. 1993 Oct;14(5):283-6.

PMID:8137410
Abstract

Standard agglutination test (SAT), enzyme-linked immunosorbent assay (ELISA), dot immunobinding assay (DIBA) have been developed for the detection of antibodies against 6 strains of Brucella and that against Yersinia enterocolitica Serotype O: g. The results showed that differentiation could be made between Brucella and Yersinia O: 9 when 16M SPA, OMP and YO: 3 SPA. OMP were used by ELISA, but not when 104M and YO: 9 antigens were used by SAT. So, 16M and YO: 3 OMP antigens were better for detection of antibodies against Brucella and Yersinia O: 9 by ELISA.

摘要

已开发出标准凝集试验(SAT)、酶联免疫吸附测定(ELISA)、斑点免疫结合测定(DIBA)用于检测针对6种布鲁氏菌菌株以及针对小肠结肠炎耶尔森菌O:9血清型的抗体。结果表明,当使用16M SPA、OMP和YO:3 SPA、OMP通过ELISA检测时,可区分布鲁氏菌和耶尔森菌O:9,但当使用SAT检测104M和YO:9抗原时则无法区分。因此,16M和YO:3 OMP抗原更适合通过ELISA检测针对布鲁氏菌和耶尔森菌O:9的抗体。

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