Eguchi M, Kikushima H, Kosaku A, Iwama Y, Sugiyama S, Sakakibara H, Ohwada Y, Sugita K, Furukawa T
Second Department of Pediatrics, Dokkyo University School of Medicine, Tochigi-ken, Japan.
Exp Mol Pathol. 1993 Dec;59(3):186-96. doi: 10.1006/exmp.1993.1038.
In order to determine whether there were any differences in distribution of nuclear DNA between acute lymphocytic leukemia (ALL) and acute myelogenous leukemia (AML), the localization of DNA in blasts from the bone marrow or buffy coat of 30 patients with ALL and 30 patients with AML was examined ultrastructurally by staining with osmium ammine B. By the ultrastructural cytochemistry, DNA in ALL cells was clumped in the nuclei, while in AML cells, it was dispersed. DNA had accumulated around the nucleoli of some blasts, and flecks of DNA were observed in nucleoli of a majority of blasts. The perinucleolar and intranucleolar DNA distribution could be classified into four types. The types with abundant perinucleolar DNA were frequently observed in ALL blasts, while the majority of AML blasts showed scant perinucleolar DNA. The types with intranucleolar flecks of DNA were more prominent in leukemic cells than in normal immature leukocytes. In conclusion, the pattern of distribution of DNA in the nuclei of leukemic cells differs between ALL and AML.
为了确定急性淋巴细胞白血病（ALL）和急性髓细胞白血病（AML）之间核DNA分布是否存在差异，采用锇胺B染色法对30例ALL患者和30例AML患者骨髓或血沉棕黄层中的原始细胞进行超微结构观察，检测DNA的定位。通过超微结构细胞化学观察发现，ALL细胞中的DNA聚集在细胞核内，而AML细胞中的DNA呈分散状态。在一些原始细胞的核仁周围有DNA积聚，并且在大多数原始细胞的核仁中观察到DNA斑点。核仁周围和核仁内的DNA分布可分为四种类型。核仁周围DNA丰富的类型在ALL原始细胞中经常观察到，而大多数AML原始细胞核仁周围的DNA较少。白血病细胞中核仁内有DNA斑点的类型比正常未成熟白细胞更为明显。总之，ALL和AML白血病细胞核内DNA的分布模式不同。
