Ohwada Y, Eguchi M
Second Department of Pediatrics, Dokkyo University School of Medicine, Tochigi-ken, Japan.
Med Oncol Tumor Pharmacother. 1993;10(3):117-24. doi: 10.1007/BF02987778.
Using DNA staining with highly stable osmium-ammine-B, the blasts of AMKL were observed under an electron microscope, in comparison with AML, ALL, CML-MK crisis and TMD. The DNA within the nucleus of the megakaryoblasts was observed as a high electron-density substance and tended to be uniformly dispersed within the nucleus. DNA, associated with nucleoli, could be roughly divided in four types based on the presence or absence of peri-nucleolar clumps and intra-nucleolar clumps. In cases of AMKL, we often observed a type that did not have peri-nucleolar DNA clumps but did have DNA flecks within the nucleoli. By analytical evaluation there were no differences among cells from several subtypes of megakaryocytic leukemia, such as AMKL in children with Down's syndrome, AMKL in children without Down's syndrome, AMKL in adults, and CML-MK crisis. The DNA distribution of TMD blasts, which were self-limited and not malignant, resembled that of AMKL blasts.
使用高稳定性锇胺-B进行DNA染色，在电子显微镜下观察急性巨核细胞白血病（AMKL）的原始细胞，并与急性髓系白血病（AML）、急性淋巴细胞白血病（ALL）、慢性粒细胞白血病-巨核细胞危象（CML-MK危机）和 transient myeloproliferative disorder（TMD）进行比较。巨核母细胞细胞核内的DNA被观察为高电子密度物质，并倾向于在细胞核内均匀分散。与核仁相关的DNA可根据核仁周围团块和核仁内团块的有无大致分为四种类型。在AMKL病例中，我们经常观察到一种类型，即没有核仁周围DNA团块，但核仁内有DNA斑点。通过分析评估，几种巨核细胞白血病亚型的细胞之间没有差异，例如唐氏综合征儿童的AMKL、非唐氏综合征儿童的AMKL、成人的AMKL以及CML-MK危机。TMD原始细胞（其为自限性且非恶性）的DNA分布与AMKL原始细胞相似。
