Ugarov V I, Jung G Y, Chetverin A B, Spirin A S
Institute of Protein Research, Russian Academy of Sciences, Puschino, Moscow Region.
FEBS Lett. 1994 Mar 14;341(1):131-4. doi: 10.1016/0014-5793(94)80255-6.
Expression of dihydrofolate reductase (DHFR) and chloramphenicol acetyltransferase (CAT) mRNAs in cell-free Escherichia coli translation systems is greatly enhanced as a result of their insertion into RQ135 RNA, a naturally occurring satellite of phage Q beta. The enhancement is due to protection of the recombinant mRNAs against endogenous ribonucleases and to an increased initial rate of translation in the case of the RQ-CAT mRNA.
