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Synergism in replication and translation of messenger RNA in a cell-free system.

作者信息

Morozov I Y, Ugarov V I, Chetverin A B, Spirin A S

机构信息

Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.

出版信息

Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9325-9. doi: 10.1073/pnas.90.20.9325.

DOI:10.1073/pnas.90.20.9325
PMID:8415701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC47560/
Abstract

Combination of the Q beta replicase reaction with the Escherichia coli cell-free translation system markedly enhances replication of a recombinant RQ-DHFR RNA consisting of the dihydrofolate reductase (DHFR) mRNA sequence inserted into RQ135(-1) RNA, an efficient naturally occurring Q beta replicase template. The enhancement is associated with a replication asymmetry previously described for the replication of Q beta phage RNA in vivo; the sense (+)-strands are produced in large excess over the antisense (-)-strands. This, in turn, results in increased synthesis of the functionally active DHFR. These effects are not observed when DHFR mRNAs or RQ135(-1) RNAs are used as templates, if the translation system is not complete, or if it is inhibited by puromycin. The coupled replication-translation of nonviral mRNA recombinants can serve as a useful model for studying the fundamental aspects of virus amplification and can be implemented for large-scale protein synthesis in vitro.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/2cb57aaf2aa6/pnas01527-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/ec1ad6d3499b/pnas01527-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/b2316d3a8c5b/pnas01527-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/f42196bc2736/pnas01527-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/2cb57aaf2aa6/pnas01527-0103-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/ec1ad6d3499b/pnas01527-0101-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/b2316d3a8c5b/pnas01527-0101-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/f42196bc2736/pnas01527-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2539/47560/2cb57aaf2aa6/pnas01527-0103-a.jpg

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1
Synergism in replication and translation of messenger RNA in a cell-free system.
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9325-9. doi: 10.1073/pnas.90.20.9325.
2
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