Tachibana A
Department of Biology, Faculty of Science, Osaka City University, Japan.
FEBS Lett. 1994 Mar 21;341(2-3):291-4. doi: 10.1016/0014-5793(94)80475-3.
A novel prenyltransferase, farnesylgeranyl diphosphate (FGPP) synthase (EC 2.5.1.X), which synthesizes C25-prenyl diphosphate, was found in the haloalkaliphilic archaeon Natronobacterium pharaonis. It was separated from geranylgeranyl diphosphate (GGPP) synthase (EC 2.5.1.29), which synthesizes C20-prenyl diphosphate, a major prenyltransferase in this organism. The highest activity of FGPP synthase was observed when GGPP was used as the allylic substrate. FGPP synthase may synthesize a precursor for the C25 moiety of C20, C25 diether lipids using a longer allylic diphosphate, such as GGPP synthesized by GGPP synthase, rather than dimethylallyl diphosphate, which is the product of isopentenyl diphosphate isomerase.
在嗜盐碱古菌法老嗜盐菌中发现了一种新型的异戊二烯基转移酶——法尼基香叶基二磷酸（FGPP）合酶（EC 2.5.1.X），它能合成C25异戊二烯基二磷酸。该酶与香叶基香叶基二磷酸（GGPP）合酶（EC 2.5.1.29）不同，后者能合成C20异戊二烯基二磷酸，是该生物体中的主要异戊二烯基转移酶。当以GGPP作为烯丙基底物时，观察到FGPP合酶的活性最高。FGPP合酶可能使用较长的烯丙基二磷酸，如由GGPP合酶合成的GGPP，而不是异戊烯基二磷酸异构酶的产物二甲基烯丙基二磷酸，来合成C20、C25二醚脂质中C25部分的前体。
