Dokras A, Ross C, Gosden B, Sargent I L, Barlow D H
Nuffield Department of Obstetrics and Gynecology, John Radcliffe Hospital, University of Oxford, United Kingdom.
Fertil Steril. 1994 Mar;61(3):514-20. doi: 10.1016/s0015-0282(16)56585-6.
To determine the effects of zona slitting and subsequent embryo transfer performed on different days of in vitro human embryonic development to allow appropriate application of assisted hatching techniques to clinical IVF-ET.
A microsurgical technique was used to make a standardized slit in the zona pellucida of bipronucleate human embryos on day 2, 3, or 5 after oocyte retrieval. A mock ET was performed after the procedure and the rate of blastocyst formation and the process of hatching in each embryo was monitored.
All embryos used were donated for research by patients attending the IVF Unit at the John Radcliffe Hospital, Oxford, United Kingdom.
The effects of the mock ET on the integrity of zona-slit embryos was documented. The embryos were cultured further and parameters such as blastocyst formation, initiation, and completion of hatching were monitored in all three groups of embryos.
The mock ET did not have a detrimental effect on embryos as assessed by their morphology and subsequent blastocyst formation rate. The technique used in this study to make an opening in the zona increased the rate and number of blastocysts hatching in all three groups compared with nonmanipulated controls. However, the day on which the slit was made did not significantly alter the outcome.
A gap in the zona pellucida of human embryos significantly increases the rate of hatching. Micromanipulative techniques such as zona slitting can be suitably used for this purpose without loss of blastomeres through the gap in the zona or trapping of the embryo during the process of hatching.
确定在人类体外胚胎发育的不同天数进行透明带切开及随后的胚胎移植，以便辅助孵化技术能恰当应用于临床体外受精 - 胚胎移植（IVF - ET）。
采用显微外科技术，在卵母细胞取出后的第2天、第3天或第5天，对双原核人类胚胎的透明带制作标准化切口。操作后进行模拟胚胎移植，并监测每个胚胎的囊胚形成率和孵化过程。
所有使用的胚胎均由英国牛津约翰拉德克利夫医院体外受精科的患者捐赠用于研究。
记录模拟胚胎移植对透明带切开胚胎完整性的影响。将胚胎进一步培养，并监测所有三组胚胎的囊胚形成、孵化起始和完成等参数。
通过胚胎形态和随后的囊胚形成率评估，模拟胚胎移植对胚胎没有不利影响。本研究中用于在透明带开口的技术与未操作的对照组相比，提高了所有三组胚胎的囊胚孵化率和囊胚数量。然而，进行切口的日期并未显著改变结果。
人类胚胎透明带中的缺口显著提高了孵化率。诸如透明带切开等显微操作技术可适用于此目的，而不会因透明带中的缺口导致卵裂球丢失或在孵化过程中使胚胎陷入困境。
