Nakayama T, Fujiwara H, Tastumi K, Fujita K, Higuchi T, Mori T
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
Fertil Steril. 1998 Apr;69(4):784-8. doi: 10.1016/s0015-0282(98)00017-x.
To estimate the efficacy of a new assisted hatching technique using a piezo-micromanipulator to support embryonic implantation.
Sibling spare embryos from human cycles of IVF were allocated to either a treated group for assisted hatching by a piezo-micromanipulator or a nontreated control group.
The Infertility and IVF unit of the Kyoto University Hospital.
PATIENT(S): Sixty-eight women undergoing conventional IVF treatment.
INTERVENTION(S): One hundred ten spare 4- or 8-cell embryos from 68 patients undergoing IVF were treated with the new assisted hatching technique, and the results were compared with those obtained for 112 sibling embryos without the treatment. In the assisted hatching procedure, zona thinning combined with drilling was performed by the vibration of a microneedle produced by the piezo-micromanipulator unit.
MAIN OUTCOME MEASURE(S): The rates of partial hatching and completely hatched blastocysts.
RESULT(S): The rates of hatching and of hatched blastocysts per total developing blastocysts were significantly higher in the treated group (86.7% and 33.3%, respectively) than in the control group (15.3% and 2.8%, respectively).
CONCLUSION(S): These results demonstrate that the newly devised zona thinning and drilling technique using a piezo-micromanipulator is useful for assisted hatching.
评估使用压电显微操作器的新型辅助孵化技术对胚胎着床的支持效果。
将体外受精（IVF）人类周期中的多余同胞胚胎分配至经压电显微操作器进行辅助孵化的治疗组或未经处理的对照组。
京都大学医院不孕不育与体外受精科。
68名接受常规IVF治疗的女性。
对68例接受IVF治疗患者的110个多余4细胞或8细胞胚胎采用新型辅助孵化技术进行处理，并将结果与112个未经处理的同胞胚胎的结果进行比较。在辅助孵化过程中，通过压电显微操作器产生的微针振动进行透明带变薄联合打孔操作。
部分孵化和完全孵化囊胚的比例。
治疗组中每个总发育囊胚的孵化率和孵化囊胚率（分别为86.7%和33.3%）显著高于对照组（分别为15.3%和2.8%）。
这些结果表明，新设计的使用压电显微操作器的透明带变薄和打孔技术对辅助孵化有用。
