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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Bermúdez Moretti M, Correa García S, Stella C, Ramos E, Batlle A M

Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Buenos Aires, Argentina.

Int J Biochem. 1993 Dec;25(12):1917-24. doi: 10.1016/0020-711x(88)90325-4.

This work represents the first approach to characterize the transport system of haem pathway precursors, such as delta-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. ALA transport occurs unidirectionally by a sole active system with an apparent KM of 0.10 mM, at the optimum pH of 5.0. ALA uptake is influenced by both the carbon and nitrogen source; this suggests a rather complex regulation mechanism. 3. This transport is not mediated by the general amino acid permease (GAP). 4. ALA uptake is strongly inhibited by compounds harboring a methyl-amine terminus suggesting that this group is essential for ALA transport; however, the electric environment of the carboxylic group may be also important for the interaction between ALA and its transporter active site. 5. We have found differences in ALA transport which would indicate a different regulation mechanism for this system in both strain cells.

本研究首次对酿酒酵母的两个菌株（野生型D27和HEM R+突变体）中血红素途径前体（如δ-氨基乙酰丙酸，ALA）的转运系统进行了表征。2. ALA通过单一的主动转运系统单向转运，表观Km值为0.10 mM，最适pH为5.0。ALA的摄取受碳源和氮源的影响；这表明存在一种相当复杂的调节机制。3. 这种转运不是由通用氨基酸通透酶（GAP）介导的。4. 带有甲胺末端的化合物能强烈抑制ALA的摄取，这表明该基团对ALA转运至关重要；然而，羧基的电荷环境对ALA与其转运体活性位点之间的相互作用可能也很重要。5. 我们发现了ALA转运的差异，这表明该系统在两种菌株细胞中的调节机制不同。

Bermúdez Moretti M, Correa García S, Stella C, Ramos E, Batlle A M

Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, F.C.E. y N., UBA), Ciudad Universitaria, Buenos Aires, Argentina.

Int J Biochem. 1993 Dec;25(12):1917-24. doi: 10.1016/0020-711x(88)90325-4.

This work represents the first approach to characterize the transport system of haem pathway precursors, such as delta-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. ALA transport occurs unidirectionally by a sole active system with an apparent KM of 0.10 mM, at the optimum pH of 5.0. ALA uptake is influenced by both the carbon and nitrogen source; this suggests a rather complex regulation mechanism. 3. This transport is not mediated by the general amino acid permease (GAP). 4. ALA uptake is strongly inhibited by compounds harboring a methyl-amine terminus suggesting that this group is essential for ALA transport; however, the electric environment of the carboxylic group may be also important for the interaction between ALA and its transporter active site. 5. We have found differences in ALA transport which would indicate a different regulation mechanism for this system in both strain cells.

本研究首次对酿酒酵母的两个菌株（野生型D27和HEM R+突变体）中血红素途径前体（如δ-氨基乙酰丙酸，ALA）的转运系统进行了表征。2. ALA通过单一的主动转运系统单向转运，表观Km值为0.10 mM，最适pH为5.0。ALA的摄取受碳源和氮源的影响；这表明存在一种相当复杂的调节机制。3. 这种转运不是由通用氨基酸通透酶（GAP）介导的。4. 带有甲胺末端的化合物能强烈抑制ALA的摄取，这表明该基团对ALA转运至关重要；然而，羧基的电荷环境对ALA与其转运体活性位点之间的相互作用可能也很重要。5. 我们发现了ALA转运的差异，这表明该系统在两种菌株细胞中的调节机制不同。