甲状腺功能减退、肾上腺切除术后以及用环己酰亚胺治疗后，棕色脂肪组织线粒体甘油磷酸酰基转移酶对冷暴露的反应。

The response of brown adipose tissue mitochondrial glycerolphosphate acyltransferase to cold-exposure in hypothyroidism, after adrenalectomy and after treatment with cycloheximide.

Mitchell J R, Saggerson E D

Department of Biochemistry & Molecular Biology, University College London, England.

Int J Biochem. 1994 Jan;26(1):67-72. doi: 10.1016/0020-711x(94)90197-x.

Abstract

Exposure to cold has previously been shown to considerably increase the activity of the mitochondrial form of glycerolphosphate acyltransferase (GPAT) in brown adipose tissue (A. C. Darnley, C. A. Carpenter and E. D Saggerson, Biochem. J. 253, 351-355, 1988; J. R. D. Mitchell and E. D. Saggerson. Biochem. J. 277, 665-669, 1991). 2. Both adrenalectomy and chemically-induced hypothyroidism increased mitochondrial GPAT activity in rats maintained at 21 degrees C. This increase was similar to that caused by exposing rats to the cold (4 degrees C) for three days. Whereas exposure of hypothyroid rats to cold (4 degrees C) resulted in a further increase in GPAT activity, no further increase in activity was observed after exposure of adrenalectomized rats to the cold. 3. Administration of triiodothyronine (T3) to rats maintained at 21 degrees C had no effect on mitochondrial GPAT activity. 4. Prior treatment with cycloheximide abolished 60-70% of the increase in GPAT activity caused by cold-exposure.

摘要

先前的研究表明，暴露于寒冷环境会显著增加棕色脂肪组织中线粒体形式的甘油磷酸酰基转移酶（GPAT）的活性（A.C. 达恩利、C.A. 卡彭特和E.D. 萨格森，《生物化学杂志》253卷，351 - 355页，1988年；J.R.D. 米切尔和E.D. 萨格森，《生物化学杂志》277卷，665 - 669页，1991年）。2. 肾上腺切除术和化学诱导的甲状腺功能减退均会增加处于21摄氏度环境中的大鼠的线粒体GPAT活性。这种增加与将大鼠暴露于寒冷（4摄氏度）环境三天所引起的增加相似。虽然甲状腺功能减退的大鼠暴露于寒冷（4摄氏度）环境会导致GPAT活性进一步增加，但肾上腺切除的大鼠暴露于寒冷环境后未观察到活性进一步增加。3. 对处于21摄氏度环境中的大鼠给予三碘甲状腺原氨酸（T3）对线粒体GPAT活性没有影响。4. 预先用环己酰亚胺处理可消除由寒冷暴露引起的GPAT活性增加的60 - 70%。