Polettini A, Groppi A, Montagna M
Institute of Legal Medicine, University of Pavia, Italy.
Forensic Sci Int. 1993 Dec;63(1-3):217-25. doi: 10.1016/0379-0738(93)90275-f.
A direct treatment of methanol-washed hair with a silylating solution is proposed to extract heroin, O-6-monoacetylmorphine, morphine, acetylcodeine, and codeine, obtaining the simultaneous derivatization of the hydroxylated metabolites and reducing potential sample contamination. Analysis is performed by capillary gas chromatography-tandem mass spectrometry (GC/MS/MS) using multiple selected reaction monitoring. Owing to the selectivity and sensitivity of the GC/MS/MS analysis, and to the extremely simple treatment of the sample, the method fulfils the requirements of both clinical and forensic diagnosis of heroin use.
提出用硅烷化溶液直接处理甲醇清洗过的毛发，以提取海洛因、O-6-单乙酰吗啡、吗啡、乙酰可待因和可待因，实现羟基化代谢物的同时衍生化并减少潜在的样品污染。采用多反应监测的毛细管气相色谱-串联质谱法（GC/MS/MS）进行分析。由于GC/MS/MS分析的选择性和灵敏度，以及样品处理极其简单，该方法满足了海洛因使用临床和法医诊断的要求。
