Jiang S, Anderson S A, Winget G D, Mukkada A J
Department of Biological Sciences, University of Cincinnati, Ohio 45221.
J Cell Physiol. 1994 Apr;159(1):60-6. doi: 10.1002/jcp.1041590109.
Leishmania donovani has an active K+/H+ exchange system on the surface membrane. Modulation of external K+ concentration resulted in a corresponding change in internal pH (pHi) suggesting a link between proton and potassium transport. Although a Na+/H+ antiporter is present on the plasma membrane, its sensitivity to amiloride suggests that it operates independent of K+/H+ exchange. Reduction of cellular ATP with NaN3 and KCN inhibits K+/H+ exchange showing thereby that the process is energy dependent. The K+/H+ exchange is sensitive to inhibitors of the gastric K+/H(+)-ATPase. It is concluded that the H(+)-ATPase previously reported on the plasma membrane of L. donovani is in fact a K+/H(+)-ATPase.
杜氏利什曼原虫在表面膜上有一个活跃的K⁺/H⁺交换系统。外部K⁺浓度的调节导致细胞内pH（pHi）相应变化，表明质子和钾运输之间存在联系。尽管质膜上存在Na⁺/H⁺反向转运体，但其对氨氯吡脒的敏感性表明它独立于K⁺/H⁺交换发挥作用。NaN₃和KCN降低细胞ATP会抑制K⁺/H⁺交换，从而表明该过程是能量依赖的。K⁺/H⁺交换对胃K⁺/H⁺-ATP酶抑制剂敏感。得出的结论是，先前报道的杜氏利什曼原虫质膜上的H⁺-ATP酶实际上是一种K⁺/H⁺-ATP酶。
