Gray D A
Department of Zoology and Entomology, Rhodes University, Grahamstown, South Africa.
J Endocrinol. 1994 Jan;140(1):85-90. doi: 10.1677/joe.0.1400085.
Polyclonal antibodies raised in a rabbit against avian atrial natriuretic peptide (ANP) were shown to reduce circulating endogenous ANP levels in Pekin ducks by more than 90%, and were subsequently used to investigate the role of this peptide in volume expansion diuresis and natriuresis. Conscious birds, undergoing a steady-state diuresis and natriuresis maintained by an i.v. infusion of hypotonic saline at a rate of 0.7 ml/min, responded to ANP antiserum (anti-ANP) with an immediate 30% reduction in urine flow rate and sodium excretion which lasted for about 30 min. Plasma arginine vasotocin levels were not changed by anti-ANP whereas circulating angiotensin II concentrations increased immediately following the administration of anti-ANP. Serum from non-immunized normal rabbits produced no changes in the renal and plasma parameters monitored. The results show that the high circulating levels of endogenous ANP associated with volume expansion promote renal salt and fluid excretion and thus have a major physiological role in avian volume homeostasis.
在兔子体内产生的针对禽心房利钠肽（ANP）的多克隆抗体，可使北京鸭体内循环的内源性ANP水平降低90%以上，随后被用于研究该肽在容量扩张性利尿和利钠中的作用。清醒的鸟类通过静脉输注以0.7毫升/分钟的速度输注低渗盐水维持稳态利尿和利钠，对ANP抗血清（抗ANP）的反应是尿流率和钠排泄立即降低30%，持续约30分钟。抗ANP对血浆精氨酸血管催产素水平无影响，而抗ANP给药后循环中的血管紧张素II浓度立即升高。未免疫的正常兔子的血清对所监测的肾脏和血浆参数无影响。结果表明，与容量扩张相关的内源性ANP的高循环水平促进肾盐和液体排泄，因此在鸟类容量稳态中具有重要的生理作用。
