Becker J L
C R Acad Hebd Seances Acad Sci D. 1975 Oct 20;281(16):1171-4.
14C-adenosine is incorporated very little inot nucleic acids of Drosophila cells cultured in a medium free of foetal calf serum. The greater part of the radioactivity is recovered in inosine and hypoxanthine. These results suggest that the principal pathway of ultilization of adenosine is not mediated by adenosine kinase. Adenosine is rapidly deaminated to inosine by adenosine deaminase, and cleaved to hypoxanthine by inosine phosphorylase. Inosine and hypoxanthine not being substrates for Drosophila cell lines, are released into the culture medium.
在不含胎牛血清的培养基中培养的果蝇细胞的核酸中，14C - 腺苷的掺入量极少。大部分放射性在肌苷和次黄嘌呤中被回收。这些结果表明，腺苷利用的主要途径不是由腺苷激酶介导的。腺苷通过腺苷脱氨酶迅速脱氨生成肌苷，并通过肌苷磷酸化酶裂解生成次黄嘌呤。由于肌苷和次黄嘌呤不是果蝇细胞系的底物，它们被释放到培养基中。
