黑长尾猴精液的冷冻保存及活动力良好精子的复苏
Cryopreservation of vervet monkey semen and recovery of progressively motile spermatozoa.
作者信息
Seier J V, Conradie E, Oettle E E, Fincham J E
机构信息
Experimental Biology Program, Primate Unit, MRC Tygerberg, South Africa.
出版信息
J Med Primatol. 1993 Aug;22(6):355-9.
PMID:8138985
Abstract
A method to cryopreserve semen from the Vervet monkey (Cercopithecus aethiops) has been developed, yielding a mean post thaw recovery of 63.60% of pre-freeze progressive motility. The extender contained a TES-TRIS buffer, egg yolk, dextrose, streptomycin, penicillin, and glycerol as cryoprotectant. The pH ranged from 7.10-7.18 and the osmolality was 330-345 mOsmol/L.
摘要
已开发出一种用于冷冻保存绿猴（长尾黑颚猴）精液的方法，解冻后平均恢复到冷冻前精子前向运动能力的63.60%。稀释液含有TES-TRIS缓冲液、蛋黄、葡萄糖、链霉素、青霉素以及作为冷冻保护剂的甘油。pH值范围为7.10 - 7.18，渗透压为330 - 345毫摩尔/升。
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