Centola G M, Raubertas R F, Mattox J H
Department of Obstetrics-Gynecology, University of Rochester Medical Center, New York 14642.
J Androl. 1992 May-Jun;13(3):283-8.
Human semen was cryopreserved using Human Sperm Preservation Medium, TEST-Yolk buffer, or glycerol alone. Sperm characteristics for each specimen were measured before and after freezing to determine which cryopreservative resulted in better cryosurvival and recovery of motile sperm. Sperm frozen in Human Sperm Preservation Medium had a significantly better recovery of all semen parameters (motility, velocity, and recovery) than either TEST-Yolk or glycerol alone. Statistical analyses also were done to examine the variability between and within donor semen specimens. Differences between donors, between specimens, and measurements within donors all contributed to variability of sperm characteristics. Specimen-to-specimen variability for a given donor represented 12% to 47% of the total variability, whereas processing and measurement variability represented 12% to 41%. Donors also varied in the ability of their sperm to tolerate freezing. There was a relationship between motile count after dilution with cryopreservative and post-thaw motile count. This relationship allows the prediction of poor-thaw survival before freezing a specimen.
使用人类精子保存培养基、TEST-卵黄缓冲液或单独使用甘油对人类精液进行冷冻保存。在冷冻前后测量每个样本的精子特征，以确定哪种冷冻保护剂能使活动精子具有更好的冷冻存活率和回收率。与单独使用TEST-卵黄或甘油相比，用人类精子保存培养基冷冻的精子在所有精液参数（活力、速度和回收率）方面的回收率明显更高。还进行了统计分析，以检查供体精液样本之间和内部的变异性。供体之间、样本之间以及供体内的测量差异均导致精子特征的变异性。给定供体的样本间变异性占总变异性的12%至47%，而处理和测量变异性占12%至41%。供体精子耐受冷冻的能力也存在差异。用冷冻保护剂稀释后的活动计数与解冻后活动计数之间存在关联。这种关系有助于在冷冻样本之前预测解冻存活率不佳的情况。
