乳腺外佩吉特病的细胞周期分析

Cell cycle analyses of extramammary Paget's disease.

作者信息

Mori O, Hachisuka H, Maeyama Y, Sasai Y

机构信息

Department of Dermatology, Kurume University School of Medicine, Japan.

出版信息

Kurume Med J. 1993;40(3):129-32. doi: 10.2739/kurumemedj.40.129.

DOI:10.2739/kurumemedj.40.129

PMID:8139211

Abstract

The cell cycle of Paget cells in extramammary Paget's disease was investigated using microscopic analysis of the DNA content, and using immunohistochemistry for 5-bromodeoxyuridine (BrdU) incorporation. Stripped skin was treated with tetrasodium ethylendiamine tetraacetate dihydrate (EDTA) and trypsin, and epidermal cells suspensions were obtained. Microscopic analysis of the DNA content in the cells was performed. The resulting DNA histogram was close to the normal ploidy. Specimens from the involved skin were incubated with BrdU, and sections were immunostained with anti-BrdU monoclonal antibody. A few nuclei showed positive staining for BrdU. The present study showed that the proliferative potential of Paget cells in extramammary Paget's disease was low. This may explain why lesions of extramammary Paget's disease enlarge rather slowly.

摘要

通过对DNA含量进行显微镜分析以及对5-溴脱氧尿苷（BrdU）掺入情况进行免疫组织化学检测，研究了乳腺外佩吉特病中佩吉特细胞的细胞周期。将剥离的皮肤用乙二胺四乙酸二钠四水合物（EDTA）和胰蛋白酶处理，获得表皮细胞悬液。对细胞中的DNA含量进行显微镜分析。所得的DNA直方图接近正常倍体。将受累皮肤的标本与BrdU一起孵育，并用抗BrdU单克隆抗体对切片进行免疫染色。少数细胞核显示BrdU阳性染色。本研究表明，乳腺外佩吉特病中佩吉特细胞的增殖潜能较低。这可能解释了为什么乳腺外佩吉特病的病变扩大得相当缓慢。