Liu F, Green M R
Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Nature. 1994 Apr 7;368(6471):520-5. doi: 10.1038/368520a0.
A puzzling property of the transcriptional activators encoded by several animal viruses is their ability to function promiscuously. The adenovirus E1a protein, for example, stimulates transcription of adenoviral genes as well as a wide variety of other viral and cellular genes. We show that E1a can interact with several classes of cellular DNA-binding domains and thereby be recruited to diverse promoters. Our results explain how a single protein can regulate transcription of multiple genes that lack a common promoter element.
几种动物病毒编码的转录激活因子有一个令人费解的特性，即它们具有广泛发挥作用的能力。例如，腺病毒E1a蛋白可刺激腺病毒基因以及多种其他病毒和细胞基因的转录。我们发现E1a能与几类细胞DNA结合结构域相互作用，从而被招募到不同的启动子上。我们的结果解释了单一蛋白质如何调控多个缺乏共同启动子元件的基因的转录。
