Chagnac A, Korzets A, Ben-Bassat M, Zevin D, Hirsh J, Meckler J, Levi J
Department of Nephrology, Hasharon Hospital, Petah-Tikva, Israel.
Nephron. 1994;66(2):176-80. doi: 10.1159/000187798.
The effects of uninephrectomy on the function and structure of the remnant kidney were assessed in rats with Adriamycin-induced nephrosis, 12 weeks after the injection of Adriamycin. The kidney volume of Adriamycin-treated uninephrectomized rats (NX-AD) was 2.3 times that of sham-operated, Adriamycin-treated animals (SH-AD; p < 0.001). The marked renal enlargement in NX-AD animals was due to the development of large tubular cysts. Following uninephrectomy, the fractional volume of tubular lumen almost doubled (NX-AD, 0.33 +/- 0.02; SH-AD, 0.17 +/- 0.02; p < 0.001) and the absolute volume of tubular lumen increased more than fourfold (NX-AD, 0.51 +/- 0.08 ml; SH-AD, 0.12 +/- 0.02 ml; p < 0.001). The frequency of tubular lumen with a large cross-sectional area (> or = 40,000 microns 2) was 5.8 +/- 1.1% in NX-AD and 0.7 +/- 0.2% in SH-AD groups (p < 0.001). The fractional volume of interstitial fibrosis in NX-AD animals was larger than in SH-AD (0.09 +/- 0.02 versus 0.04 +/- 0.01%, p < 0.05). As opposed to the worsening of tubulointerstitial disease, single-kidney glomerular filtration rate, fractional protein clearance, glomerular volume and the extent of glomerular sclerosis did not differ significantly in NX-AD as compared to SH-AD groups. This study shows that uninephrectomy in rats with Adriamycin nephrosis worsens interstitial nephrosis and aggravates the formation of tubular cysts, leading to a macrocystic kidney disease. These changes are not associated with an increase in glomerular sclerosis.
在注射阿霉素12周后，对阿霉素诱导的肾病大鼠进行单侧肾切除，评估其对残余肾脏功能和结构的影响。经阿霉素处理的单侧肾切除大鼠（NX - AD）的肾脏体积是假手术、经阿霉素处理动物（SH - AD）的2.3倍（p < 0.001）。NX - AD动物明显的肾脏肿大是由于大的肾小管囊肿形成。单侧肾切除后，肾小管腔的分数体积几乎翻倍（NX - AD，0.33 ± 0.02；SH - AD，0.17 ± 0.02；p < 0.001），肾小管腔的绝对体积增加了四倍多（NX - AD，0.51 ± 0.08 ml；SH - AD，0.12 ± 0.02 ml；p < 0.001）。横截面积大（≥40,000平方微米）的肾小管腔频率在NX - AD组为5.8 ± 1.1%，在SH - AD组为0.7 ± 0.2%（p < 0.001）。NX - AD动物的间质纤维化分数体积大于SH - AD组（0.09 ± 0.02对0.04 ± 0.01%，p < 0.05）。与肾小管间质疾病恶化相反，与SH - AD组相比，NX - AD组的单肾肾小球滤过率、分数蛋白清除率、肾小球体积和肾小球硬化程度没有显著差异。这项研究表明，阿霉素肾病大鼠的单侧肾切除会使间质性肾病恶化，并加重肾小管囊肿的形成，导致大囊性病。这些变化与肾小球硬化的增加无关。
