Sharma S C, Barry-Kinsella C, Cottell E, Harrison R F
Department of Pharmacology, Trinity College, Dublin, Ireland.
Prostaglandins. 1994 Jan;47(1):9-16. doi: 10.1016/0090-6980(94)90070-1.
The study was carried out following the approval of our Hospital Ethics Committee and an informed patient consent. Samples of peritoneal fluid were collected from the Pouch of Douglas at laparoscopy from 32 subjects with minimal stage endometriosis (MSE) and 16 without any visible signs of endometriosis (normal group). All the subjects were in 20 to 23 day period of their menstrual cycle which was histologically confirmed. The endometriosis score was estimated according to the American Fertility Score, 1985 and the fluid samples were collected in tubes containing heparin/indomethacin and standing in ice. The samples were estimated for volume, erythrocyte count, prostaglandin(PG) F2 alpha, and PGE2. Radioimmunoassay techniques were used for the estimation of PGs. Prostaglandin F2 alpha, was measured in its parent form while PGE2 was converted into bicyclic-PGE2 before estimation. The results indicate that the peritoneal fluid in women with MSE has higher than normal levels of PGF2 alpha and lower than normal levels of PGE2 and its PGE2: PGF2 alpha ratio is also below normal. There is no difference in the volume of peritoneal fluid in the two groups. Provided the fluid sample is not contaminated with abdominal blood its erythrocyte count is not related to its prostaglandin content.
本研究在获得我院伦理委员会批准并取得患者知情同意后开展。在腹腔镜检查时，从Douglas陷凹采集32例轻度子宫内膜异位症（MSE）患者及16例无任何子宫内膜异位症可见体征患者（正常组）的腹腔液样本。所有受试者均处于月经周期的第20至23天，经组织学证实。根据1985年美国生育评分系统评估子宫内膜异位症评分，并将腹腔液样本收集于含肝素/消炎痛的试管中，置于冰上。对样本进行体积、红细胞计数、前列腺素（PG）F2α和PGE2的测定。采用放射免疫分析技术测定前列腺素。以前列腺素F2α的母体形式进行测量，而PGE2在测定前转化为双环PGE2。结果表明，MSE女性患者腹腔液中PGF2α水平高于正常，PGE2水平低于正常，且其PGE2:PGF2α比值也低于正常。两组腹腔液体积无差异。若腹腔液样本未被腹腔血液污染，其红细胞计数与其前列腺素含量无关。
