Karck U, Reister F, Schäfer W, Zahradnik H P, Breckwoldt M
Department of Obstetrics and Gynecology, Freiburg University Hospital, Germany.
Prostaglandins. 1996 Jan;51(1):49-60. doi: 10.1016/0090-6980(95)00159-x.
To test for differences in the amount and activity of peritoneal macrophages present in the peritoneal fluid of women with, and without endometriosis using prostaglandin release by macrophages in culture as a marker.
Women of reproductive age undergoing laparoscopy for infertility or chronic pelvic pain with postoperative diagnosis of endometriosis and women undergoing laparoscopy for sterilization.
Peritoneal fluid was aspirated during laparascopy, volume was recorded, macrophages were isolated via a Ficoll Paque gradient and kept in primary culture. PGE2 and PGF2 alpha release of the cells were measured before and after stimulation with zymosan.
Women with endometriosis had significantly more peritoneal macrophages than controls. Peritoneal macrophages of women with endometriosis released significantly more PGE2 than those of the control group: 8.4 +/- 2.0 versus 1.4 +/- 0.4 ng/ml/10(6) cells (mean +/- SEM, p = 0.0005) and PGF2 alpha: 10 +/- 4.3 (endometriosis) versus 1.8 +/- 0.4 (control) ng/ml/10(6) cells (mean +/- SEM, p = 0.045).
There is a significant increase in the amount of prostaglandins released by peritoneal macrophages from women with endometriosis. These prostaglandins might alter uterine and tubal contractility, thereby affecting fertility.
以培养的巨噬细胞释放前列腺素作为标志物，检测患有和未患有子宫内膜异位症的女性腹膜液中腹膜巨噬细胞数量及活性的差异。
因不孕或慢性盆腔疼痛接受腹腔镜检查且术后诊断为子宫内膜异位症的育龄期女性，以及因绝育接受腹腔镜检查的女性。
在腹腔镜检查期间抽取腹膜液，记录体积，通过Ficoll Paque梯度分离巨噬细胞并进行原代培养。在用酵母聚糖刺激前后测量细胞的前列腺素E2（PGE2）和前列腺素F2α（PGF2α）释放量。
患有子宫内膜异位症的女性腹膜巨噬细胞数量显著多于对照组。患有子宫内膜异位症的女性腹膜巨噬细胞释放的PGE2显著多于对照组：8.4±2.0与1.4±0.4纳克/毫升/10⁶个细胞（平均值±标准误，p = 0.0005），PGF2α：10±4.3（子宫内膜异位症组）与1.8±0.4（对照组）纳克/毫升/10⁶个细胞（平均值±标准误，p = 0.045）。
患有子宫内膜异位症的女性腹膜巨噬细胞释放的前列腺素量显著增加。这些前列腺素可能会改变子宫和输卵管的收缩力，从而影响生育能力。
