Nilsson B, Theodorsson E, Jivegård L, Thune A, Friman S, Svanvik J
Department of Surgery, Sahlgrenska Hospital, Göteborg, Sweden.
Regul Pept. 1994 Jan 13;49(3):179-84. doi: 10.1016/0167-0115(94)90139-2.
The inflammatory fluid secretion by the gallbladder mucosa in experimental cholecystitis is induced by an increased prostaglandin formation and is mediated by intramural nerves. In the present study the effect of VIP-antiserum on the inflammatory fluid secretion in the gallbladder was tested in a validated experimental model in cats. The animals were studied in acute experiments 6 weeks after a procedure when the cystic duct was tied and gallstones were implanted in the gallbladder. During basal conditions there was a continuous secretion of fluid into the lumen of the inflamed gallbladder averaging 0.43 +/- 0.18 ml/h. Injection of VIP antiserum, obtained from immunized rabbits and diluted with saline 1:10 in a bolus of 4 ml into the coeliac artery reversed this secretion into an absorption of 1.72 +/- 0.44 ml h-1 (P < 0.001). VIP-antiserum did not affect the fluid adsorption in control animals with an intact gallbladder and injection of control serum from rabbits not immunized to VIP did not affect fluid secretion in the inflamed gallbladders. The results support the idea that the inflammatory fluid secretion in the gallbladder mucosa is mediated by VIP-ergic nerve fibres.
实验性胆囊炎时胆囊黏膜的炎性液体分泌是由前列腺素生成增加所诱导，并由壁内神经介导。在本研究中，在一个经过验证的猫实验模型中测试了血管活性肠肽（VIP）抗血清对胆囊炎性液体分泌的影响。在一项手术6周后的急性实验中对动物进行研究，该手术为结扎胆囊管并在胆囊内植入胆结石。在基础状态下，有液体持续分泌到发炎胆囊的腔内，平均为0.43±0.18毫升/小时。将从免疫兔获得并以1:10用生理盐水稀释成4毫升大剂量注入腹腔动脉的VIP抗血清，使这种分泌逆转成1.72±0.44毫升/小时的吸收（P<0.001）。VIP抗血清对胆囊完整的对照动物的液体吸收没有影响，并且注射未免疫VIP的兔的对照血清对发炎胆囊的液体分泌也没有影响。这些结果支持这样一种观点，即胆囊黏膜的炎性液体分泌是由VIP能神经纤维介导的。
