Jivegård L, Fahrenkrug J, Svanvik J
Department of Surgery, Sahlgrenska Hospital, University of Gõteborg, Sweden.
Regul Pept. 1989 May;25(2):179-85. doi: 10.1016/0167-0115(89)90259-0.
Intravenous infusion of vasoactive intestinal peptide (VIP) causes gallbladder mucosal fluid secretion by an action on epithelial cell receptors in the cat. Gallbladder fluid secretion is observed also in experimental cholecystitis and this secretion is abolished when the intramural gallbladder nerves are blocked. In the present study, immunoreactive VIP was detected in the gallbladder contents (29 +/- 5 (S.E.M.) pM) in the obstructed lumen of the gallbladder in cats with experimental cholecystitis and gallbladder mucosal fluid secretion, but not in the normal feline gallbladder. During luminal perfusion of the gallbladder in vivo, the calculated secretion of VIP into the gallbladder lumen in animals with experimental cholecystitis was significantly higher (0.31 +/- 0.08 (S.E.M.), pmol/h) than in controls (0.11 +/- 0.02 (S.E.M.), pmol/h) while plasma levels of VIP were similar. Recovery of exogenously administered VIP was similar in normal and inflamed gallbladders. The present results support the hypothesis that intramural VIP-releasing nerve fibers may be activated in cholecystitis.
静脉输注血管活性肠肽（VIP）可通过作用于猫胆囊上皮细胞受体引起胆囊黏膜分泌液体。在实验性胆囊炎中也观察到胆囊液体分泌，当胆囊壁内神经被阻断时，这种分泌就会消失。在本研究中，在患有实验性胆囊炎且有胆囊黏膜液体分泌的猫的胆囊梗阻腔内的胆囊内容物中检测到免疫反应性VIP（29±5（标准误）pM），但在正常猫胆囊中未检测到。在体内对胆囊进行腔内灌注时，患有实验性胆囊炎的动物胆囊腔内VIP的计算分泌量（0.31±0.08（标准误），pmol/小时）显著高于对照组（0.11±0.02（标准误），pmol/小时），而血浆中VIP水平相似。正常胆囊和发炎胆囊对外源性给予的VIP的回收率相似。目前的结果支持这样的假设，即在胆囊炎中胆囊壁内释放VIP的神经纤维可能被激活。
