Ferrándiz F, Ródenas S, Villegas A
Departamento de Nutrición y Bromatología II: Bromatología y Técnicas Analíticas Farmacéuticas, Facultad de Farmacia, Universidad Complutense, Madrid.
Sangre (Barc). 1993 Oct;38(5):393-7.
Seven patients with hereditary spherocytosis have been studied using polyacrylamide gel electrophoresis with sodium dodecylsulfate (PAGE-SDS) and cytopherometry, in order to obtain information about possible alterations in the erythrocyte membrane proteins and in the electrophoretic mobility of whole erythrocytes. In four patients, a decrease in Band 4.2 protein was found. Histogram plotters proved of interest in showing two subpopulations in two patients. In all the cases, the electrophoretic mobility was normal. In two patients a spectrin deficiency was found. The study of histograms showed the presence of two subpopulations in this group of patients, in which the electrophoretic mobility was normal. Finally, one patient showed no deficiencies in membrane proteins. This fact can be due to an abnormality of spectrin that we could not detect with our techniques.
为了获取有关红细胞膜蛋白和全红细胞电泳迁移率可能变化的信息，我们使用十二烷基硫酸钠聚丙烯酰胺凝胶电泳（PAGE-SDS）和细胞光度测定法对7例遗传性球形红细胞增多症患者进行了研究。在4例患者中，发现4.2带蛋白减少。直方图绘图仪在显示2例患者中的两个亚群方面很有用。在所有病例中，电泳迁移率均正常。在2例患者中发现了血影蛋白缺乏。对直方图的研究表明，在这组患者中存在两个亚群，其电泳迁移率正常。最后，1例患者未显示膜蛋白缺乏。这一事实可能是由于我们的技术无法检测到的血影蛋白异常所致。
