Berry I J, Burns J E, Parkinson E K
Beaston Institute for Cancer Research, CRC Beaston Laboratories, Glasgow, Scotland.
Mol Carcinog. 1994 Mar;9(3):134-42. doi: 10.1002/mc.2940090305.
Two human cell lines derived from squamous cell carcinomas (SCCs) of the epidermis, SCC-12 clone F and SCC-13 clone Y, were made to be independent of the Swiss 3T3 feeder layer to perform somatic-cell genetic experiments. We fused these SCC lines with normal human fibroblasts, and all resulting hybrids senesced after completing 12-17 population doublings, suggesting that in part, immortalization of the keratinocyte during SCC development results from the loss of gene function. We also tested whether these two SCC lines mapped to known complementation groups for immortality by fusing them with representatives of groups A (GM847), B (HeLa), and C (143B), but most of these hybrids were indistinguishable from those derived from homotypic crosses set up as immortal hybrid controls. As reported by others, fusions of cell lines from different complementation groups-143B (group C) x HeLa (group B) or GM847 (group A) x Hela (group B)--resulted in predominantly senescent hybrids. Our results confirmed and extended previous observations by others that the phenomenon of senescence is dominant to that of immortality, but they did not allow us to assign either of the SCC lines we studied to a complementation group for immortality.
从表皮鳞状细胞癌（SCC）衍生而来的两个人类细胞系，即SCC - 12克隆F和SCC - 13克隆Y，被培育成不依赖于瑞士3T3饲养层，以进行体细胞遗传学实验。我们将这些SCC细胞系与正常人成纤维细胞融合，所有产生的杂种细胞在完成12 - 17次群体倍增后衰老，这表明在SCC发展过程中角质形成细胞的永生化部分是由于基因功能丧失所致。我们还通过将这两个SCC细胞系与A组（GM847）、B组（HeLa）和C组（143B）的代表细胞融合，来测试它们是否映射到已知的永生互补组，但这些杂种细胞大多与作为永生杂种对照而建立的同型杂交产生的细胞无法区分。正如其他人所报道的，来自不同互补组的细胞系融合——143B（C组）×HeLa（B组）或GM847（A组）×HeLa（B组）——主要产生衰老的杂种细胞。我们的结果证实并扩展了其他人之前的观察结果，即衰老现象比永生现象更具主导性，但我们无法将所研究的任何一个SCC细胞系归入永生互补组。
