Moy E L, Duncan E L, Hukku B, Reddel R R
Cancer Research Unit, Children's Medical Research Institute, Sydney, NSW, Australia.
Exp Gerontol. 1997 Nov-Dec;32(6):663-70. doi: 10.1016/s0531-5565(97)00092-2.
Previous somatic cell hybridization studies have assigned many human cell lines to one of four complementation groups (A-D) for immortalization. We report here that the A1698DM cell line, which contains selectable markers and has previously been defined as the immortalization group D representative, was derived from T24 cells rather than A1698. A1698DM did not undergo senescence when fused with cell lines assigned to groups A, B, or C. This raises the possibility that this cell line has undergone further evolution and lost multiple putative senescence genes so that it is now unable to complement any, or most, other cell lines for senescence. Cell lines previously assigned to group D may, therefore, be heterogeneous with respect to the genetic changes that resulted in their immortalization. This has important implications for strategies to clone senescence genes based on complementation groups.
先前的体细胞杂交研究已将许多人类细胞系归类为四个永生化互补组（A - D）之一。我们在此报告，A1698DM细胞系含有可选择标记，此前被定义为永生化D组的代表，它是源自T24细胞而非A1698。A1698DM与分配到A、B或C组的细胞系融合时不会发生衰老。这增加了一种可能性，即该细胞系经历了进一步进化并丢失了多个假定的衰老基因，以至于现在它无法与任何其他细胞系或大多数其他细胞系在衰老方面互补。因此，先前分配到D组的细胞系在导致其永生化的基因变化方面可能是异质的。这对于基于互补组克隆衰老基因的策略具有重要意义。
