Mineura K, Izumi I, Kuwahara N, Kowada M
Neurological Service, Akita University Hospital, Japan.
Acta Oncol. 1994;33(1):29-32. doi: 10.3109/02841869409098371.
The activity of O6-methylguanine-DNA methyltransferase (O6-MT), which removes O6-methyl residues from O6-methylguanine-DNA leading to cell death, has been reported to correlate with sensitivity to nitrosoureas used for chemotherapy of gliomas. We determined O6-MT activity in tumors and matched brain tissue from patients with gliomas. Histological diagnoses were: six malignant astrocytomas, two glioblastomas, two oligodendrogliomas, one ependymoma, and one medulloblastoma. In all cases but one, the activity ranged widely from 39 to 258 fmol/mg protein extract. The wide range of activity of the tumor tissue may indicate varying degree of sensitivity to nitrosoureas. The activity of brain tissue, available from the peritumoral region of five cases, varied between 38 to 415 fmol/mg. Four of the five regions showed a higher value than the respective tumor, and one showed a lower value.
O6-甲基鸟嘌呤-DNA甲基转移酶（O6-MT）可从O6-甲基鸟嘌呤-DNA中去除O6-甲基残基，从而导致细胞死亡，据报道，该酶的活性与用于胶质瘤化疗的亚硝基脲的敏感性相关。我们测定了胶质瘤患者肿瘤组织及配对脑组织中的O6-MT活性。组织学诊断结果为：6例恶性星形细胞瘤、2例胶质母细胞瘤、2例少突胶质细胞瘤、1例室管膜瘤和1例髓母细胞瘤。除1例之外，所有病例中该酶活性范围为39至258 fmol/mg蛋白提取物，差异较大。肿瘤组织活性范围较宽可能表明对亚硝基脲的敏感程度不同。5例患者瘤周区域脑组织的活性在38至415 fmol/mg之间。5个区域中有4个区域的值高于相应肿瘤组织，1个区域的值低于肿瘤组织。
