Montero R, Gonsebatt M E, Herrera L A, Rojas E, Ostrosky-Wegman P
Instituto de Investigaciones Biomédicas, UNAM, México D. F.
Environ Health Perspect. 1993 Oct;101 Suppl 3(Suppl 3):135-8. doi: 10.1289/ehp.93101s3135.
This paper reviews several monitoring studies where the short-term HPRT assay has been applied. The original method uses autoradiography to detect 3H-thymidine incorporation in variant cells that have undergone DNA synthesis; the bromodeoxyuridine modification employs this thymidine analog and fluorescence plus Giemsa staining. The studies discussed here were accomplished with either of these methods. methods. Exposures analyzed include radiation and chemotherapy as medical treatments and accidental exposures to radiation; these studies have been useful in the validation of the assay because radiation and anticancer drugs are well-known mutagens. Other potential mutagens such as environmental arsenic and a parasitic infection and praziquantel, used for its treatment, have also been monitored for hprt locus mutation. An overview of the results obtained with different agents and routes of exposure is presented here as well as some methodological aspects for the optimization of the assay for monitoring studies.
本文回顾了几项应用短期次黄嘌呤鸟嘌呤磷酸核糖转移酶（HPRT）检测法的监测研究。原始方法使用放射自显影术来检测已进行DNA合成的变异细胞中3H-胸腺嘧啶核苷的掺入；溴脱氧尿苷修饰法使用这种胸腺嘧啶类似物以及荧光加吉姆萨染色。这里讨论的研究是用这两种方法中的任何一种完成的。分析的暴露包括作为医学治疗的辐射和化疗以及意外辐射暴露；这些研究在检测法的验证中很有用，因为辐射和抗癌药物是众所周知的诱变剂。其他潜在诱变剂，如环境中的砷、一种寄生虫感染以及用于治疗的吡喹酮，也已针对HPRT基因座突变进行了监测。本文介绍了不同试剂和暴露途径所获得结果的概述，以及监测研究检测法优化的一些方法学方面。
