Ostrosky-Wegman P, Gonsebatt M E, Montero R, Vega L, Barba H, Espinosa J, Palao A, Cortinas C, García-Vargas G, del Razo L M
Instituto de Investigaciones Biomédicas, UNAM, Mexico, D.F.
Mutat Res. 1991 Sep-Oct;250(1-2):477-82. doi: 10.1016/0027-5107(91)90204-2.
In the search for early biological markers to detect genetic damage, a pilot study on a hydroarsenicism-exposed group was designed. Blood and urine samples were taken from 11 individuals chronically exposed and from 13 individuals with lower exposure to the metal. Lymphocyte cultures for cytogenetic studies and HGPRT assay were done with coded peripheral blood samples, while arsenic levels and the "rec assay" in B. subtilis were determined in urine samples. The highly exposed group excreted greater amounts of As, nevertheless the rec assay showed negative results. An interesting finding is that the cell-cycle kinetics exhibited a significant difference between the groups studied, the average generation time (AGT) was longer in the highly exposed group. The percentages of chromosomal aberrations and the frequencies of sister-chromatid exchanges were similar in both populations, although complex aberrations were more frequent in the highly exposed group, which also showed a higher average variation frequency in the HGPRT assay, but the 2 latter observations were not statistically significant. The lag in lymphocyte proliferation could mean an impairment of the immune response due to arsenic exposure.
为了寻找检测遗传损伤的早期生物学标志物，设计了一项针对砷中毒暴露组的初步研究。采集了11名长期暴露者和13名低暴露者的血液和尿液样本。用编码外周血样本进行淋巴细胞培养以进行细胞遗传学研究和次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶（HGPRT）测定，同时在尿液样本中测定砷含量和枯草芽孢杆菌中的“rec测定”。高暴露组排出的砷量更多，但rec测定结果为阴性。一个有趣的发现是，所研究的组之间细胞周期动力学存在显著差异，高暴露组的平均世代时间（AGT）更长。两个群体中的染色体畸变百分比和姐妹染色单体交换频率相似，尽管复杂畸变在高暴露组中更频繁，并且在HGPRT测定中该组也显示出更高的平均变异频率，但后两个观察结果无统计学意义。淋巴细胞增殖滞后可能意味着砷暴露导致免疫反应受损。
