Akgöl S, Dinçkaya E
Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova-Izmir, Turkey.
Talanta. 1999 Feb;48(2):363-7.
A biosensor for the specific determination of hydrogen peroxide was developed using catalase (EC 1.11.1.6) in combination with a dissolved oxygen probe. Catalase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane served as enzyme electrode. The electrode response was maximum when 50 mM phosphate buffer was used at pH 7.0 and at 35 degrees C. The biosensor response depends linearly on hydrogen peroxide concentration between 1.0x10(-5) and 3.0x10(-3) M with a response time of 30 s. The sensor is stable for >3 months so in this period >400 assays can be performed.
开发了一种用于特异性测定过氧化氢的生物传感器，该传感器使用过氧化氢酶（EC 1.11.1.6）与溶解氧探头相结合。过氧化氢酶通过戊二醛与明胶固定，并固定在经过预处理的聚四氟乙烯膜上作为酶电极。当在pH 7.0和35℃下使用50 mM磷酸盐缓冲液时，电极响应最大。生物传感器响应在1.0×10（-5）至3.0×10（-3）M的过氧化氢浓度范围内呈线性关系，响应时间为30秒。该传感器在超过3个月的时间内稳定，因此在此期间可进行超过400次测定。
