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信号肽在蓝细菌中蛋白质靶向定位中的作用。

Role of signal peptides in targeting of proteins in cyanobacteria.

作者信息

Mackle M M, Zilinskas B A

机构信息

Department of Plant Science, Cook College, Rutgers University, New Brunswick, New Jersey 08903-0231.

出版信息

J Bacteriol. 1994 Apr;176(7):1857-64. doi: 10.1128/jb.176.7.1857-1864.1994.

DOI:10.1128/jb.176.7.1857-1864.1994
PMID:8144451
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205287/
Abstract

Proteins of cyanobacteria may be transported across one of two membrane systems: the typical eubacterial cell envelope (consisting of an inner membrane, periplasmic space, and an outer membrane) and the photosynthetic thylakoids. To investigate the role of signal peptides in targeting in cyanobacteria, Synechococcus sp. strain PCC 7942 was transformed with vectors carrying the chloramphenicol acetyltransferase reporter gene fused to coding sequences for one of four different signal peptides. These included signal peptides of two proteins of periplasmic space origin (one from Escherichia coli and the other from Synechococcus sp. strain PCC 7942) and two other signal peptides of proteins located in the thylakoid lumen (one from a cyanobacterium and the other from a higher plant). The location of the gene fusion products expressed in Synechococcus sp. strain PCC 7942 was determined by a chloramphenicol acetyltransferase enzyme-linked immunosorbent assay of subcellular fractions. The distribution pattern for gene fusions with periplasmic signal peptides was different from that of gene fusions with thylakoid lumen signal peptides. Primary sequence analysis revealed conserved features in the thylakoid lumen signal peptides that were absent from the periplasmic signal peptides. These results suggest the importance of the signal peptide in protein targeting in cyanobacteria and point to the presence of signal peptide features conserved between chloroplasts and cyanobacteria for targeting of proteins to the thylakoid lumen.

摘要

蓝细菌的蛋白质可能通过两种膜系统之一进行转运

典型的真细菌细胞包膜（由内膜、周质空间和外膜组成）和光合类囊体。为了研究信号肽在蓝细菌靶向中的作用，用携带氯霉素乙酰转移酶报告基因的载体转化了聚球藻属菌株PCC 7942，该报告基因与四种不同信号肽之一的编码序列融合。这些信号肽包括两种源于周质空间的蛋白质的信号肽（一种来自大肠杆菌，另一种来自聚球藻属菌株PCC 7942）以及另外两种位于类囊体腔中的蛋白质的信号肽（一种来自蓝细菌，另一种来自高等植物）。通过对亚细胞组分进行氯霉素乙酰转移酶酶联免疫吸附测定，确定了在聚球藻属菌株PCC 7942中表达的基因融合产物的位置。与周质信号肽的基因融合的分布模式不同于与类囊体腔信号肽的基因融合的分布模式。一级序列分析揭示了类囊体腔信号肽中存在而周质信号肽中不存在的保守特征。这些结果表明信号肽在蓝细菌蛋白质靶向中的重要性，并指出在叶绿体和蓝细菌之间存在保守的信号肽特征，用于将蛋白质靶向类囊体腔。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3546/205287/8ac203ffdae7/jbacter00025-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3546/205287/40131602093f/jbacter00025-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3546/205287/8ac203ffdae7/jbacter00025-0064-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3546/205287/40131602093f/jbacter00025-0062-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3546/205287/8ac203ffdae7/jbacter00025-0064-a.jpg

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