Yashiro K, Sakamoto T, Ohmori M
Department of Biology, Graduate School of Arts and Sciences, University of Tokyo, Japan.
Plant Mol Biol. 1996 Apr;31(1):175-81. doi: 10.1007/BF00020618.
A cyaA gene, encoding an adenylate cyclase, was isolated from a filamentous cyanobacterium, Spirulina platensis, by functional complementation of a cya mutant of Escherichia coli, defective in adenylate cyclase activity. The predicted gene product of cyaA contains a signal peptide-like domain, a putative sensor domain similar to the gene product of vsrA of Pseudomonas solanacearum, a putative membrane-spanning domain and an adenylate cyclase-like catalytic domain. Two other positive clones that complemented the E. coli mutant were isolated from the same cyanobacterium, suggesting that several cya genes are functioning in S. platensis.
通过对缺乏腺苷酸环化酶活性的大肠杆菌cya突变体进行功能互补，从丝状蓝细菌钝顶螺旋藻中分离出一个编码腺苷酸环化酶的cyaA基因。cyaA基因的预测产物包含一个信号肽样结构域、一个与青枯雷尔氏菌vsrA基因产物相似的假定传感结构域、一个假定的跨膜结构域和一个腺苷酸环化酶样催化结构域。从同一蓝细菌中分离出另外两个能互补大肠杆菌突变体的阳性克隆，这表明钝顶螺旋藻中有几个cya基因在发挥作用。
