Dempsey J A, Cannon J G
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.
J Bacteriol. 1994 Apr;176(7):2055-60. doi: 10.1128/jb.176.7.2055-2060.1994.
To increase the utility of the previously constructed physical map of the chromosome of Neisseria gonorrhoeae FA1090, 28 additional genetic markers were localized on the map. Cloned gonococcal genes were used to probe Southern blots of restriction enzyme-digested DNA separated on pulsed-field gels, thus identifying the fragment in each of several digests to which the probe hybridized and the map location of each gene. The addition of the new markers brings the total number of mapped loci for this strain to 68; the locations of all of those markers on the updated map are shown.
为提高先前构建的淋病奈瑟菌FA1090染色体物理图谱的实用性，在该图谱上定位了另外28个遗传标记。用克隆的淋球菌基因探测经脉冲场凝胶分离的限制性内切酶消化DNA的Southern杂交印迹，从而确定每个探针杂交的几种消化产物中的片段以及每个基因的图谱位置。新标记的加入使该菌株的定位基因座总数达到68个；图中显示了所有这些标记在更新图谱上的位置。
