类似于小鼠Crry/p65的大鼠细胞膜补体抑制剂。
Complement inhibitor of rat cell membrane resembling mouse Crry/p65.
作者信息
Takizawa H, Okada N, Okada H
机构信息
Department of Molecular Biology, Nagoya City University School of Medicine, Japan.
出版信息
J Immunol. 1994 Mar 15;152(6):3032-8.
Neuraminidase-treated rat erythrocytes are resistant to homologous rat C although they are readily hemolyzed by heterologous serum C via the alternative pathway. We established a mAb, mAb512, which allows for hemolysis of neuraminidase-treated rat erythrocytes by homologous rat serum. mAb512 detected rat erythrocyte membrane components with molecular masses of 65 kDa and 55 kDa by Western blotting analysis. Furthermore, mAb512 caused C3b deposition on rat myeloma cells after treatment with rat serum. 512Ag was purified by use of an immunosorbent column prepared with mAb512. Partial sequencing of 512Ag peptides showed significant homology to mouse Crry/p65 indicating that 512Ag could be the rat counterpart of mouse Crry/p65.
经神经氨酸酶处理的大鼠红细胞对同源大鼠补体C具有抗性，尽管它们很容易通过替代途径被异源血清补体溶血。我们制备了一种单克隆抗体mAb512，它能使经神经氨酸酶处理的大鼠红细胞被同源大鼠血清溶血。通过蛋白质印迹分析，mAb512检测到分子质量为65 kDa和55 kDa的大鼠红细胞膜成分。此外，mAb512在用大鼠血清处理后可导致大鼠骨髓瘤细胞上C3b沉积。使用用mAb512制备的免疫吸附柱纯化了512Ag。对512Ag肽段的部分测序显示与小鼠Crry/p65有显著同源性，表明512Ag可能是小鼠Crry/p65的大鼠对应物。
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