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鱿鱼突触前终末中钙信号的空间分布。

The spatial distribution of calcium signals in squid presynaptic terminals.

作者信息

Smith S J, Buchanan J, Osses L R, Charlton M P, Augustine G J

机构信息

Department of Molecular Physiology, Beckman Center, Stanford University Medical School, Palo Alto, CA 94305-5425.

出版信息

J Physiol. 1993 Dec;472:573-93. doi: 10.1113/jphysiol.1993.sp019963.

DOI:10.1113/jphysiol.1993.sp019963
PMID:8145162
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1160503/
Abstract
  1. The fluorescent Ca2+ indicator dye, fura-2, was used to examine the spatial distribution of intracellular Ca2+ signals in giant presynaptic terminals of squid. Brief trains of presynaptic action potentials were evoked to open Ca2+ channels within the giant presynaptic terminals and elevate presynaptic Ca2+ concentration. 2. Electrical stimulation produced pronounced rises in presynaptic Ca2+ concentration. These rises were much larger in the terminal region than in the adjacent axonal region of the presynaptic neuron, suggesting that Ca2+ channels are most abundant in the terminal. 3. Stimulation also produced gradients in Ca2+ concentration across the width of the presynaptic terminal. During stimulation, Ca2+ concentration was highest in the compartment of the presynaptic terminal closest to the postsynaptic neuron. This suggests that the Ca2+ channels are localized to this region of the presynaptic terminal. 4. Following the end of action potential trains, the rises in Ca2+ concentration became uniform across the width of the terminal. The redistribution of Ca2+ presumably is due to diffusion of Ca2+ throughout the presynaptic cytoplasm. Stimulus-evoked rises in Ca2+ declined slowly over several tens of seconds. 5. Histological examination of a giant presynaptic terminal used for imaging experiments revealed that the spatial compartments where stimulus-induced rises in Ca2+ concentration were highest were also enriched in active zones, the presynaptic sites of transmitter secretion. The co-localization of Ca2+ transients and active zones strongly suggests that neurons cluster Ca2+ channels selectively at active zones and that they do so to enhance the magnitude of Ca2+ signals in the vicinity of the active zone. 6. Longitudinal gradients in Ca2+ concentration also occur within presynaptic terminals and can be quantitatively accounted for by gradients in surface/volume ratio and density of active zones along the length of the presynaptic terminal.
摘要

  1. 荧光钙指示剂染料fura - 2被用于检测鱿鱼巨大突触前终末内细胞内钙信号的空间分布。诱发短暂的突触前动作电位序列，以打开巨大突触前终末内的钙通道并提高突触前钙浓度。

  2. 电刺激使突触前钙浓度显著升高。这些升高在终末区域比突触前神经元相邻的轴突区域大得多，这表明钙通道在终末最为丰富。

  3. 刺激还在突触前终末的宽度上产生了钙浓度梯度。在刺激期间，钙浓度在突触前终末最靠近突触后神经元的隔室内最高。这表明钙通道定位于突触前终末的该区域。

  4. 在动作电位序列结束后，钙浓度的升高在终末宽度上变得均匀。钙的重新分布大概是由于钙在突触前细胞质中扩散所致。刺激诱发的钙升高在几十秒内缓慢下降。

  5. 对用于成像实验的一个巨大突触前终末进行组织学检查发现，刺激诱导的钙浓度升高最高的空间隔室也富含活性区，即递质分泌的突触前位点。钙瞬变与活性区的共定位强烈表明，神经元在活性区选择性地聚集钙通道，并且这样做是为了增强活性区附近钙信号的幅度。

  6. 钙浓度的纵向梯度也出现在突触前终末内，并且可以通过沿突触前终末长度的表面/体积比和活性区密度的梯度进行定量解释。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/61353577e2b9/jphysiol00414-0584-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/19f0339f1737/jphysiol00414-0575-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/94434d1d8f79/jphysiol00414-0577-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/294cc0e4779b/jphysiol00414-0582-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/61353577e2b9/jphysiol00414-0584-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/19f0339f1737/jphysiol00414-0575-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/94434d1d8f79/jphysiol00414-0577-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/294cc0e4779b/jphysiol00414-0582-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f4c/1160503/61353577e2b9/jphysiol00414-0584-a.jpg

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