Nakagawa H, Uchida Y, Takamura E, Nakagawa Y, Araki H, Watanabe M
Department of Ophthalmology, Tokyo Women's Medical College, Japan.
Jpn J Ophthalmol. 1993;37(4):505-13.
Herpes simplex virus (HSV) antigens in corneal or conjunctival epithelial lesions were detected by impression cytology. Specimens were obtained using a nitrocellulose membrane and were stained by the peroxidase-antiperoxidase method. HSV antigens were demonstrated in 30 of 32 patients with herpes simplex keratitis or conjunctivitis. Impression specimens of dendritic or stellate keratitis lesions exhibited precise replicas of corneal lesions with numerous antigen-positive cells. HSV antigens were also detectable in some of the minute stellate and/or punctate epithelial keratitis lesions even during the course of antiviral treatment. Impression cytology is noninvasive and useful for a rapid etiological diagnosis of herpetic epithelial lesions.
采用印片细胞学方法检测角膜或结膜上皮病变中的单纯疱疹病毒（HSV）抗原。使用硝酸纤维素膜获取标本，并采用过氧化物酶-抗过氧化物酶法进行染色。32例单纯疱疹性角膜炎或结膜炎患者中，有30例检测到HSV抗原。树枝状或星状角膜炎病变的印片标本显示出角膜病变的精确复制品，有大量抗原阳性细胞。即使在抗病毒治疗过程中，一些微小的星状和/或点状上皮角膜炎病变中也可检测到HSV抗原。印片细胞学是非侵入性的，有助于对疱疹性上皮病变进行快速病因诊断。
