Cussó R, Domingo A, Rosell-Pérez M
Rev Esp Fisiol. 1975 Dec;31(4):309-16.
A decrease in glycogen content and glycogen-synthetase activity was observed in leukemic leucocytes compared to those of normal humans, but the decrease was lesser in chronic myeloid leukemic leucocytes than in those of acute leukemic patients. Incubation of crude leucocyte homogenates at 30 degrees C before assay of the enzyme activity, revealed a conversion of the glycogen-synthetase from the D to the I form. It took place if the enzyme source were enzyme extracts from acute myeloblastic, acute lymphoblastic, chronic lymphocytic, acute menoblastic and undifferenciate leucocytes. The conversion did not occur in crude homogenates from leucocytes of chronic myeloid leukemic patients. ATP-Mg caused a conversion of the glycogen-synthetase I form to the D form in acute myeloblastic, chronic lymphocytic and undifferenciate leukemic leucocytes. This conversion was not observed in chronic myelocytic leukemic leucocytes.
与正常人的白细胞相比，白血病白细胞中的糖原含量和糖原合成酶活性降低，但慢性粒细胞白血病白细胞的降低程度小于急性白血病患者。在测定酶活性之前，将粗白细胞匀浆在30℃孵育，发现糖原合成酶从D型转变为I型。如果酶源是来自急性髓细胞性、急性淋巴细胞性、慢性淋巴细胞性、急性单核细胞性和未分化白细胞的酶提取物，就会发生这种转变。慢性粒细胞白血病患者白细胞的粗匀浆中未发生这种转变。ATP-Mg可使急性髓细胞性、慢性淋巴细胞性和未分化白血病白细胞中的糖原合成酶I型转变为D型。在慢性粒细胞白血病白细胞中未观察到这种转变。
