Obika M, Fukuzawa T
Department of Biology, Keio University, Yokohama, Japan.
Pigment Cell Res. 1993 Dec;6(6):417-22. doi: 10.1111/j.1600-0749.1993.tb00624.x.
Cytoskeletal construction of dermal chromatophores of Oryzias latipes was studied by immunofluorescence microscopy. A microtubule system was most prominent in melanophores where a large number of microtubules emanated from the center of the cell. Xanthophores had an arrangement basically similar to that of melanophores, though the radial pattern became more irregular in the peripheral region where intersecting wavy microtubules were quite frequent. Oval-shaped leucophores exhibited the least-developed microtubule system, where the limited number of microtubules formed a loose basket-like architecture. Intermediate filaments were ubiquitously present in all types of chromatophores and were found to be vimentin-immunoreactive. Examination of doubly-labeled cells indicated that vimentin filaments had similar distribution patterns with microtubules. Orderly arranged bundles of actin filaments were found only in xanthophores, while in melanophores and xanthophores, actin expression was diffuse without displaying a conspicuous filamentous organization. Colchicine treatment induced depolymerization of microtubules and retraction of dendrites in varying degrees in cells in culture and in situ. Melanophores in culture are very sensitive to the treatment while xanthophores appeared to be more resistant in respect to the maintenance of cell morphology.
利用免疫荧光显微镜对青鳉真皮色素细胞的细胞骨架结构进行了研究。微管系统在黑素细胞中最为显著，大量微管从细胞中心发出。黄色素细胞的排列方式与黑素细胞基本相似，不过在周边区域，放射状模式变得更加不规则，相交的波浪状微管相当频繁。椭圆形的白色素细胞显示出最不发达的微管系统，其中数量有限的微管形成了松散的篮状结构。中间丝普遍存在于所有类型的色素细胞中，并且被发现对波形蛋白呈免疫反应。对双重标记细胞的检查表明，波形蛋白丝与微管具有相似的分布模式。仅在黄色素细胞中发现了排列有序的肌动蛋白丝束，而在黑素细胞和黄色素细胞中，肌动蛋白表达呈弥漫性，未显示出明显的丝状结构。秋水仙碱处理在培养细胞和原位细胞中不同程度地诱导了微管解聚和树突回缩。培养中的黑素细胞对该处理非常敏感，而黄色素细胞在维持细胞形态方面似乎更具抗性。
