Iwata A, Iwata M, Nakano E
Cell Struct Funct. 1986 Jun;11(2):99-107. doi: 10.1247/csf.11.99.
Distributions of fibronectin, microtubules and microfilaments in the melanophore of the medaka, Oryzias latipes, were studied using immunofluorescence. We found fluorescent fibers of fibronectin running from the central region to the periphery of the melanophore, but patterns differed depending on whether melanophores had been treated with methanol. Fibrous structures were present in methanol-treated melanophores, whereas only diffuse fluorescence was seen in untreated ones. These fibrous structures, thought to be microtubules, were arranged radially. Actin fibers also showed a radial arrangement, but were not as well organized. In addition, granules with actin immunofluorescence were present.
利用免疫荧光技术研究了青鳉（Oryzias latipes）黑素细胞中纤连蛋白、微管和微丝的分布情况。我们发现纤连蛋白的荧光纤维从黑素细胞的中央区域延伸至周边区域，但根据黑素细胞是否用甲醇处理，其模式有所不同。甲醇处理过的黑素细胞中存在纤维状结构，而未处理的黑素细胞中只观察到弥散荧光。这些被认为是微管的纤维状结构呈放射状排列。肌动蛋白纤维也呈放射状排列，但排列不如微管有序。此外，还存在具有肌动蛋白免疫荧光的颗粒。
