Gyoeva F K, Leonova E V, Rodionov V I, Gelfand V I
Institute of Protein Research Academy of Science of the USSR, Pushchino, Moscow Region.
J Cell Sci. 1987 Dec;88 ( Pt 5):649-55. doi: 10.1242/jcs.88.5.649.
The distribution and chemical composition of intermediate filaments in cultured melanophores of two teleost species - Gymnocorymbus ternetzi and Pterophyllum scalare - were studied by immunofluorescence staining and immunoblotting techniques. The immunofluorescence staining of the melanophores with monoclonal and polyclonal antibodies to the intermediate filament protein vimentin revealed a system of fibrils radiating from the cell centre. These fibrils were resistant to 0.6 M-KCl and nocodazole treatments as has been found in other cell types. Transmission electron microscopy confirmed the presence of intermediate filaments in melanophores. Immunoblotting experiments showed the presence of the intermediate filament protein vimentin in melanophore lysates. Therefore, teleost melanophores possess a developed radial system of vimentin intermediate filaments.
通过免疫荧光染色和免疫印迹技术，研究了两种硬骨鱼——三色魮脂鲤和神仙鱼——培养的黑素细胞中中间丝的分布和化学成分。用针对中间丝蛋白波形蛋白的单克隆抗体和多克隆抗体对黑素细胞进行免疫荧光染色，结果显示有一个从细胞中心辐射出来的纤维系统。正如在其他细胞类型中所发现的那样，这些纤维对0.6M - KCl和诺考达唑处理具有抗性。透射电子显微镜证实了黑素细胞中存在中间丝。免疫印迹实验表明黑素细胞裂解物中存在中间丝蛋白波形蛋白。因此，硬骨鱼黑素细胞拥有一个发达的波形蛋白中间丝辐射系统。
