Pollock J M, McNair J, Kennedy S, Kennedy D G, Walsh D M, Goodall E A, Mackie D P, Crockard A D
Veterinary Sciences Division, Department of Agriculture for Northern Ireland, Stormont, Belfast.
Res Vet Sci. 1994 Jan;56(1):100-7. doi: 10.1016/0034-5288(94)90203-8.
Four groups of calves depleted of alpha-tocopherol and selenium (Se) were supplemented with alpha-tocopherol or Se or alpha-tocopherol and Se or received no supplement. In vitro lymphocyte proliferative responses were measured in fetal calf serum (FCS), in autologous serum and in pooled sera from each group. In FCS, the responses to pokeweed mitogen were significantly enhanced for calves supplemented with alpha-tocopherol. In autologous serum, the mean responses to keyhole limpet haemocyanin (KLH) were greatest for calves supplemented with Se alone. In pooled sera from each group, lymphocytes from calves supplemented with Se alone showed enhanced responses to KLH in the presence of serum from calves supplemented with alpha-tocopherol. The calves depleted of alpha-tocopherol had increased circulating percentages of BoCD2 lymphocytes, apparently due to changes in the BoCD4 subpopulation. The percentages of B cells were greatest in calves supplemented with alpha-tocopherol and Se. The results indicate that alpha-tocopherol and Se have interactive effects on lymphocyte responses to antigen and suggest that micronutrient status is important when interpreting the results of in vitro assays of lymphocyte function.
四组缺乏α-生育酚和硒（Se）的犊牛分别补充α-生育酚或硒或α-生育酚和硒，或不进行补充。在胎牛血清（FCS）、自体血清以及每组的混合血清中测量体外淋巴细胞增殖反应。在FCS中，补充α-生育酚的犊牛对商陆有丝分裂原的反应显著增强。在自体血清中，单独补充硒的犊牛对钥孔戚血蓝蛋白（KLH）的平均反应最大。在每组的混合血清中，单独补充硒的犊牛的淋巴细胞在补充α-生育酚的犊牛的血清存在下对KLH的反应增强。缺乏α-生育酚的犊牛循环中BoCD2淋巴细胞的百分比增加，这显然是由于BoCD4亚群的变化。B细胞的百分比在补充α-生育酚和硒的犊牛中最高。结果表明，α-生育酚和硒对淋巴细胞对抗原的反应有交互作用，并表明在解释淋巴细胞功能的体外试验结果时，微量营养素状态很重要。
