Dzhankov I, Sumrov I, Lozeva T
Vet Med Nauki. 1975;12(4):29-33.
An antigen analysis of Borrelia anserina strains, belonging to two serotypes, was carried out for the first time by means of direct immunofluorescence microscopy, gel diffusion after Ouchterlony, immunoelectrophoresis as modified by Scheidegger, and whole serum macroagglutination reaction. It was found that the spirochetes of the strain Rouen of Pamoukchii serotype possess eight surface antigens, and those of strain Surnevo I of the same serotype have 6. The two strains were found to have as many as four common surface antigens, on the one hand, and two type-specific antigens each, on the other. Strain Rouen has a heat-resistant type-specific antigen, probably of a polysaccharide nature. Two of the surface antigens of strain Rouen were found to be more deeply bound in strain Surnevo I.
首次通过直接免疫荧光显微镜检查、奥克特洛尼(Ouchterlony)凝胶扩散、经谢德格(Scheidegger)改良的免疫电泳以及全血清宏观凝集反应,对属于两种血清型的鹅疏螺旋体菌株进行了抗原分析。结果发现,帕穆基(Pamoukchii)血清型鲁昂(Rouen)菌株的螺旋体具有8种表面抗原,同一血清型的苏尔涅沃一世(Surnevo I)菌株有6种。一方面,发现这两种菌株有多达4种共同的表面抗原,另一方面,每种菌株又有两种型特异性抗原。鲁昂菌株有一种耐热的型特异性抗原,可能具有多糖性质。还发现鲁昂菌株的两种表面抗原在苏尔涅沃一世菌株中结合得更深。